human codon optimized streptococcus pyogenes cas9 gene (Addgene inc)
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human codon optimized streptococcus pyogenes cas9 gene
Human Codon Optimized Streptococcus Pyogenes Cas9 Gene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 369 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+codon+optimized+cas9+gene/SP-Cas9+(Plasmid+%2362731)/pmc08399046__mmc2-222-9-30
Average 96 stars, based on 369 article reviews
Human Codon Optimized Streptococcus Pyogenes Cas9 Gene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 369 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+codon+optimized+cas9+gene/SP-Cas9+(Plasmid+%2362731)/pmc08399046__mmc2-222-9-30
Average 96 stars, based on 369 article reviews
human codon optimized streptococcus pyogenes cas9 gene - by Bioz Stars,
2026-10
96/100 stars
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Plasmid Preparation:Article Title: SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae Article Snippet: .. To generate the different types of cas9 gene fragments for integration into the X-3 locus, the Article Title: Comparison of CRISPR/Cas9 and TALENs on editing an integrated EGFP gene in the genome of HEK293FT cells Article Snippet: Briefly, intermediary arrays were produced for Golden Gate cloning as follows; 150 ng each pFUS_A, pFUS_B, pLR-X and pC- + 63-TAL modified vector were incubated for 10 cycles of 5 min at 37 °C and 10 min at 16 °C, then heated to 50 °C for 5 min and then 80 °C for 5 min in the presence of 50 units T4 DNA ligase (New England Biolabs), 10 units Esp3I (Fermentas), 1 × T4 ligase buffer (New England Biolabs) (TALEN RVD sequences in Additional file : Table 1). .. The plasmid encoding Article Title: Induction of site-specific chromosomal translocations in embryonic stem cells by CRISPR/Cas9. Article Snippet: .. Article Title: Comparison of CRISPR/Cas9 and TALENs on editing an integrated EGFP gene in the genome of HEK293FT cells. Article Snippet: Briefly, intermediary arrays were produced for Golden Gate cloning as follows; 150 ng each pFUS_A, pFUS_B, pLR-X and pC- + 63-TAL modified vector were incubated for 10 cycles of 5 min at 37 °C and 10 min at 16 °C, then heated to 50 °C for 5 min and then 80 °C for 5 min in the presence of 50 units T4 DNA ligase (New England Biolabs), 10 units Esp3I (Fermentas), 1 × T4 ligase buffer (New England Biolabs) (TALEN RVD sequences in Additional file 6: Table 1). .. The plasmid encoding Polymerase Chain Reaction:Article Title: SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae Article Snippet: .. To generate the different types of cas9 gene fragments for integration into the X-3 locus, the Amplification:Article Title: SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae Article Snippet: .. To generate the different types of cas9 gene fragments for integration into the X-3 locus, the Generated:Article Title: Comparison of CRISPR/Cas9 and TALENs on editing an integrated EGFP gene in the genome of HEK293FT cells Article Snippet: Briefly, intermediary arrays were produced for Golden Gate cloning as follows; 150 ng each pFUS_A, pFUS_B, pLR-X and pC- + 63-TAL modified vector were incubated for 10 cycles of 5 min at 37 °C and 10 min at 16 °C, then heated to 50 °C for 5 min and then 80 °C for 5 min in the presence of 50 units T4 DNA ligase (New England Biolabs), 10 units Esp3I (Fermentas), 1 × T4 ligase buffer (New England Biolabs) (TALEN RVD sequences in Additional file : Table 1). .. The plasmid encoding Article Title: Comparison of CRISPR/Cas9 and TALENs on editing an integrated EGFP gene in the genome of HEK293FT cells. Article Snippet: Briefly, intermediary arrays were produced for Golden Gate cloning as follows; 150 ng each pFUS_A, pFUS_B, pLR-X and pC- + 63-TAL modified vector were incubated for 10 cycles of 5 min at 37 °C and 10 min at 16 °C, then heated to 50 °C for 5 min and then 80 °C for 5 min in the presence of 50 units T4 DNA ligase (New England Biolabs), 10 units Esp3I (Fermentas), 1 × T4 ligase buffer (New England Biolabs) (TALEN RVD sequences in Additional file 6: Table 1). .. The plasmid encoding Clone Assay:Article Title: Induction of site-specific chromosomal translocations in embryonic stem cells by CRISPR/Cas9. Article Snippet: .. Expressing:Article Title: Induction of site-specific chromosomal translocations in embryonic stem cells by CRISPR/Cas9. Article Snippet: .. |
